IMAGE

Fig. 2

ID

ZDB-IMAGE-250315-50

Genes

tbk1

Publication

Raas et al., 2025 - TBK1 is involved in programmed cell death and ALS-related pathways in novel zebrafish models

All Figures

Figures for Raas et al., 2025

Image

Figure Caption

Fig. 2 Tbk1 expression and survival in zebrafish larvae with CRISPR-induced deletion of the tbk1 gene.

A Description of CRISPR-Cas9 tandem sgRNA targeting exons 1 and 2 or exons 4 and 5 of the tbk1 gene (top) and genotyping of injected embryos with gel electrophoresis of PCR products amplifying the deleted region. B tbk1 protein quantification by mass spectrometry in zebrafish larvae harboring mosaic tbk1 mutations or their noninjected controls (NIs) (unpaired t test, *p < 0.05). C RT‒qPCR measurement of tbk1 expression in zebrafish larvae with mosaic deletions of the tbk1 gene or NI controls between 6 hpf and 8 dpf (unpaired t test, ***p < 0.001). D Survival rate of zebrafish larvae injected with tbk1 or mismatch AMO (ns), CRISPR-Cas9 sgRNA targeting exons 1 and 2 or exons 4 and 5 of the tbk1 gene or NI controls (log-rank (Mantel‒Cox) test, *** p < 0.001).

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Death Discov