Fig. 3

Combinatorial expression of t-type marker genes separates subtypes along the anatomical axes of the OT.

a, atf5b-expressing cells were labelled in three individual larvae and patterns were coregistered (total n = 2,312 cells). b, Combinatorial expression of marker genes within the atf5b-expressing cells. atf5b⁺ etv1⁺ cells were found in the deep SPV region, whereas atf5b⁺cebpa⁺ cells were localized to the superficial region with highest density towards the posterior zone. c, scRNA-seq data for clusters expressing atf5b. d, sp5l-expressing cells were labelled from 3 individual larvae (total n = 2,058). e, Combinatorial expression of marker genes among the sp5l-expressing cells. sp5l⁺chata⁺ cells were found in the intermediate SPV region. sp5l⁺neurod1⁺ cells were found in the intermediate and deep regions, with highest density towards the posterior zone. sp5l⁺ uts1⁺ cells represented a rare population located in the intermediate SPV region. f, scRNA-seq data for clusters expressing sp5l.