FIGURE

Fig. 4

ID

ZDB-FIG-250217-17

Publication

Spampinato et al., 2025 - Lactate accumulation promotes immunosuppression and fibrotic transformation of bone marrow microenvironment in myelofibrosis

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Fig. 4

TPOhigh zebrafish as an in vivo model of megakaryocyte expansion and fibrosis development. (A) A schematic view of TPOhigh zebrafish model. (B) Flow cytometry analysis of WKM in control and TPOhigh zebrafish. (C) Evaluation of the percentage of each blood lineage after TPO treatment. (D) May-Gr�nwald Giemsa images of hematopoietic cells isolated from WKM of control and TPOhigh zebrafish. Scale bar: 5 ?m. (E) qPCR analysis of megakaryocytic markers and (F) col1a1 and tgfb1 expression in WKM. B2m was used as housekeeping gene. Calculated value of 2^???Ct in control was 1. (G) Representative immunofluorescence images of Col1A1 and (H) immunohistochemical images of reticulum fibers in control and TPOhigh zebrafish. Scale bar: 40 ?m; magnification: 40X. *p?

Expression Data

Genes:	col1a1a itga2b mpl tgfb1a
Fish:	AB
Condition:	chemical treatment by environment: eltrombopag
Anatomical Term:	head kidney
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB
Condition:	chemical treatment by environment: eltrombopag
Observed In:	head kidney head kidney erythroid lineage cell head kidney hematopoietic cell head kidney myeloid cell
Stage:	Adult

Phenotype Detail

Acknowledgments

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