FIGURE

Fig. 5

ID

ZDB-FIG-250203-44

Publication

Shi et al., 2025 - An animal model recapitulates human hepatic diseases associated with GATA6 mutations

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

The coordinated interactions among Gata6, Hhex, and Lrh-1 modulate the output of ?-catenin signaling. (A) WISH showing myca expression at 30 hpf. Red arrowhead, liver bud. (B) Immunostaining of Tg(Tp1:GFP) embryos with or without XAV939 treatment (18 hpf-54 hpf) at 3.5 dpf using Bhmt antibody (red, hepatocytes) (n = 10/10, DMSO, n = 11/14 XAV939). (Scale bar, 50 ?m.) (C) Immuno- and dye staining of livers in the siblings (n = 14) and prt mutants (n = 12) at 5.5 dpf. (Scale bar, 50 ?m.) (D) TOP-FLASH assays wherein Lrh-1 and the increasing amounts of Gata6 expression in HEK293T cells. (E) Coimmunoprecipitation assays of Gata6 and Lrh-1 in HEK293T cells. (F) Luciferase assays of the lrh-1 promoter with Gata6, Hhex, and Lrh-1 in HepG2 cells. (G) Immunoassay of Flag-Gata6 and HA-Hhex with or without CHX treatment at the indicated time points in HEK293T cells. (H) A model to depict the molecular mechanism underlying liver development in zebrafish. Data are mean � SD, ****P < 0.0001, two-tailed unpaired t test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA