Fig. 1
AlphaFold-Multimer predicts the formation of a trimeric complex (A) Outline of the AlphaFold-Multimer (v2)37,38 screen predicting putative protein-protein interactions of Izumo1, Spaca6, Dcst1, and Dcst2, with ∼1,400 testis-expressed secreted and membrane proteins. (B) Violin plot of AlphaFold-Multimer ipTM scores split by bait (interface-predicted template modeling [ipTM] score). The pairwise predictions between Izumo1, Spaca6, and Tmem81 are highlighted. (C) Phylogenetic protein tree of Tmem81 (red), Izumo1 (yellow), and Spaca6 (blue). See Figure S1A for extended tree. (D) Tertiary structure AlphaFold236 predictions of zebrafish Tmem81, Izumo1, and Spaca6 ectodomains with flexible linkers and transmembrane helices as schemes. (E–G) Predicted structural models of the trimeric sperm complex in zebrafish (E) and mice (G). Ectodomains are shown in two orientations. Predicted aligned error (PAE) plots (F) of the top-scoring models of the zebrafish (top) and mouse (bottom) trimeric complexes containing the full-length mature proteins. PAE is a confidence measure for the relative position of any two residues within the predicted structure. Index: amino acid residues. See also Figures S1 and S2. |
Reprinted from Cell, 187(25), Deneke, V.E., Blaha, A., Lu, Y., Suwita, J.P., Draper, J.M., Phan, C.S., Panser, K., Schleiffer, A., Jacob, L., Humer, T., Stejskal, K., Krssakova, G., Roitinger, E., Handler, D., Kamoshita, M., Vance, T.D.R., Wang, X., Surm, J.M., Moran, Y., Lee, J.E., Ikawa, M., Pauli, A., A conserved fertilization complex bridges sperm and egg in vertebrates, 7066-7078.e22, Copyright (2024) with permission from Elsevier. Full text @ Cell