FIGURE

Figure 6

ID

ZDB-FIG-250109-277

Publication

Dai et al., 2024 - Hyperaminoacidemia from interrupted glucagon signaling increases pancreatic acinar cell proliferation and size via mTORC1 and YAP pathways

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Figure 6

Yap1 is required for mTORC1 activation in gcgr−/− fish

(A and B) Quantification of the percentage of EdU-labeled acinar cells and the acinar cell size in the pancreas sections WT, gcgr−/−, and gcgr−/− with yap1 knockdown.

(C) Representative immunofluorescence images of pS6(240/244) in pancreas sections. All fish carry the Tg(ela3l:EGFP) transgene that labels acinar cells with EGFP (scale bar, 10 μm).

(D) Quantification of raw pS6 signal intensity in acinar cells of these fish.

(E) Quantification of the percentage of pS6-positive acinar cells in the pancreas sections. n = 7, 6, 8 for each group). Data are represented as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. One-way ANOVA followed by Tukey’s multiple comparisons test.

(F) Proposed model of IGS-induced acinar cell hyperplasia and hypertrophy.

Expression Data

Antibody:	Ab9-rps6
Fish:	gz2Tg gcgra^vu600/vu600; gcgrb^vu601/vu601; gz2Tg gcgra^vu600/vu600; gcgrb^vu601/vu601; gz2Tg + CRISPR13-yap1 + CRISPR14-yap1
Knockdown Reagents:	CRISPR13-yap1 CRISPR14-yap1
Anatomical Term:	pancreatic acinar cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gcgra^vu600/vu600; gcgrb^vu601/vu601 gcgra^vu600/vu600; gcgrb^vu601/vu601; gz2Tg gcgra^vu600/vu600; gcgrb^vu601/vu601 + CRISPR13-yap1 + CRISPR14-yap1 gcgra^vu600/vu600; gcgrb^vu601/vu601; gz2Tg + CRISPR13-yap1 + CRISPR14-yap1
Knockdown Reagents:	CRISPR13-yap1 CRISPR14-yap1
Observed In:	acinar cell proliferation pancreatic acinar cell
Stage:	Adult

Phenotype Detail

Acknowledgments

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