FIGURE

Figure 3

ID

ZDB-FIG-250109-274

Publication

Dai et al., 2024 - Hyperaminoacidemia from interrupted glucagon signaling increases pancreatic acinar cell proliferation and size via mTORC1 and YAP pathways

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Figure 3

Slc38a5 mediates acinar cell growth

(A) The top 11 amino acid transporters expressed in mouse acinar cells (n = 5, data from RNA-seq).

(B) Slc38a5 expression in acinar cells of IgG and GCGR-Ab treated mice. CPM, count per million. Student’s t test.

(C) Representative images of zebrafish pancreas from control, gcgr−/−, and gcgr−/− with slc38a5b knockdown groups. Green, amylase; red, EdU; blue, DAPI. Scale bar, 10 μm.

(D) Quantification of EdU+ acinar cells in control, gcgr−/−, and gcgr−/− with slc38a5b knockdown group (n = 16/genotype).

(E) Acinar cell size in control, gcgr−/−, and gcgr−/− with slc38a5b knockdown groups. Each data point is the average of more than 50 cells from the same fish. Data are represented as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. One-way ANOVA followed by Turkey’s Multiple Comparisons Test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gcgra^vu600/vu600; gcgrb^vu601/vu601 gcgra^vu600/vu600; gcgrb^vu601/vu601 + CRISPR3-slc38a5b + CRISPR4-slc38a5b
Knockdown Reagents:	CRISPR3-slc38a5b CRISPR4-slc38a5b
Observed In:	acinar cell proliferation pancreatic acinar cell
Stage:	Adult

Phenotype Detail

Acknowledgments

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