Fig. 7

The abnormal motor behavior in zebrafish larvae induced by BPA is alleviated by FA through the ROS/JNK pathway. (A-D) The levels of ROS were measured in zebrafish larvae at 120 hpf using a DCFH-DA fluorescent probe (10 μM). The red dotted regions indicate areas where the fluorescence intensity was statistically analyzed to determine ROS levels (n = 9). (E-G) The levels of LPO (nmol/mg protein), H2O2 (μmol/g), and CAT (units/mg protein) were also measured in zebrafish larvae at 5 dpf (n = 100). (H-J) The inhibitory effects of FA and NAC on the activation of JNK/p-JNK protein levels in zebrafish larvae at 5 dpf were observed (n = 50). BPA, 30 μM; FA, 50 μM; NAC, 5 μM. (K-M) FA and NAC were found to have a synergistic effect in rescuing abnormal motor behavior, movement distance, and average movement speed of zebrafish larvae at 5 dpf after exposure to BPA (n = 18). BPA, 30 μM; FA, 50 μM; NAC, 5 μM. The data are presented as mean ± standard error. Statistical significance was denoted as *P<0.05, **P<0.01, ***P<0.001, and ****P<0.0001.