FIGURE

Fig. 6

ID

ZDB-FIG-250106-14

Publication

Dernovšek et al., 2024 - First dual inhibitors of human topoisomerase IIα and Hsp90 C-terminal domain inhibit the growth of Ewing sarcoma in vitro and in vivo

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Fig. 6

Insights into the cytostatic and cytotoxic behavior of compound 3. (A) Time dependency of apoptosis with corresponding representative experiments measuring the changes in proportion of Annexin V and/or SytoxBlue positive SK-N-MC cells (right). The bar graph represents means and SD of at least two independent experiments (left). (B) Effect of compound 3 on Hsp90 client protein c-Raf and heat shock proteins Hsp70 and Hsp90 in Ewing sarcoma cell line SK-N-MC after 24 h of treatment. Full images used for quantification are shown in Supporting information Fig. S9. (C) Schematic representation of the workflow applied for the in vivo evaluation of compound 3 and 17-DMAG in zebrafish larvae xenograft model of Ewing sarcoma. (D) Graphical depiction of a statistically significant inhibition of increase in relative change in tumor size in larvae treated with 1 % DMSO vs. 17-DMAG (30 µM) vs. compound 3 (10 µM); Error bars represent mean ± SEM. Statistical analysis was performed with a Mann Whitney test (**p < 0.01).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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