Figure 3

Pyrazinib-functionalised gold nanoparticles (AuNP-P3) significantly altered the levels of secreted mediators in response to ionizing radiation in an OAC cell line model of radioresistance. The secreted levels of protein in OE33P (radiation sensitive) and OE33R (radiation resistant) cells were evaluated by 54-plex ELISA. Here, we report data only for the significantly modulated proteins. (A) AuNP-P3 10 µM significantly alters the level of 9 proteins (IL-2, IL-7, IL-12p40, IL12-p70, IL-13, IL-16, IL1β, MCP-1, and sICAM-1) in OE33P cells (radiosensitive) when compared to the control (0.1% DMSO). (B) AuNP-P3 10 µM significantly alters the level of 5 proteins (IL-2, IL-10, IL12-p70, IL-16, and IL1β) in OE33R cells (radioresistant) when compared to the control (0.1% DMSO + 0.1% water). Two-tailed paired t-test; * p < 0.05, ** p < 0.01, *** p < 0.001, # p < 0.05 (P3 vs. AuNP-P3) (n = 3). Data are expressed as mean ± SEM (n = 3). (C) Correlation plots showing significant correlations between the levels of secreted proteins and the metabolic rates in OE33P and OE33R cells treated with AuNP-P3 10 µM in the presence or absence of 2 Gy radiation. (Spearman correlation, blue indicates positive correlations and red indicates inverse negative correlations). The Holm–Bonferroni hoc correction was used to control for multiple comparison testing. * p < 0.05, ** p < 0.01, *** p < 0.001.