Figure 2

Pyrazinib-functionalised gold nanoparticles (AuNP-P3) treatment reduced oxidative phosphorylation and glycolysis in response to ionizing radiation in an OAC cell line model of radioresistance. OE33P (radiation sensitive cells) and OE33R (radiation resistant cells) were treated for 24 h with the compounds at 10 µM or with the control (0.1% DMSO + 0.1% water) and subsequently irradiated at 2 Gy X-ray radiation. After 24 h, cells were used to measure metabolic rates and the supernatants were stored for multiplex ELISA. Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were measured in real-time using Seahorse Biosciences XFe24 analyser. Comparison of basal and radiation-induced OCR (A) and ECAR (B) levels between OE33P and OE33R cells. Relative percentage OCR (C,D) and ECAR (E,F) in OE33P cells following treatment with compounds at 10 μM and mock or 2 Gy radiation. Relative percentage OCR (G,H) and ECAR (I,J) in OE33R cells following treatment with compounds at 10 μM and mock or 2 Gy radiation. Data are expressed as mean ± SEM. Statistical analysis was carried out using an unpaired t-test to compare different cell lines and paired t-test to compare within the same cell line. * p < 0.05, ** p < 0.01 (Compound vs. Control); # p < 0.05 (AuNP-P3 vs. AuNPs) (n = 3).