Electroretinogram (ERG) results of efemp1+/+ and efemp12C-Cas9 fish under normal rearing. (A,B,C,D) Group average ERG traces with shades areas indicating SEM, (A1,B1,C1,D1) b-wave amplitudes, (A2,B2,C2,D2) a-wave amplitudes, (A3,B3,C3,D3) b-wave implicit times and (A4,B4,C4,D4) a-wave implicit times at 2 (A–A4, respectively), 4 (B–B4, respectively), 6 (C–C4, respectively) and 8 (D–D4, respectively) weeks post-fertilization (wpf). There were 14 and 11 eyes for 2 wpf, 13 and 12 eyes for 4 wpf, 20 and 17 eyes for 6 wpf, and 17 and 16 eyes for 8 wpf efemp1+/+ and efemp12C-Cas9 fish, respectively. Scale bars: 300 µV in A and B and 100 µV in C and D. Group data are shown as mean±s.e.m. Two-way ANOVA and Fisher's LSD post-hoc tests were performed. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001.
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