Fig. 5.

Electroretinography (ERG) of efemp1^+/+ and efemp1^2C-Cas9 fish after 2 days, 2 weeks or 4 weeks of L/D or D/D rearing. (A–H) Group average ERG traces for (A) L/D-reared efemp1^+/+ (n=12), (B) L/D-reared efemp1^2C-Cas9 (n=13), (C) D/D-reared efemp1^+/+ (n=13) and (D) D/D-reared efemp1^2C-Cas9 eyes (n=14). Shaded areas indicated SEM. Group (E) b-wave amplitude, (F) a-wave amplitude, (G) b-wave implicit time and (H) a-wave implicit times measured after 2 days of treatment. Vertical scale bar indicates 100 µV and horizontal scale bar represents 100 ms in A–D. (I–P) Group average ERG traces for (I) L/D-reared efemp1^+/+ (n=14), (J) L/D-reared efemp1^2C-Cas9 (n=15), (K) D/D-reared efemp1^+/+ (n=16) and (L) D/D-reared efemp1^2C-Cas9 eyes (n=15), as well as their (M) b-wave amplitude, (N) a-wave amplitude, (O) b-wave implicit time and (P) a-wave implicit time measured after 2 weeks of treatment. (Q–X) Group average ERG traces for (Q) L/D-reared efemp1^+/+ (n=15), (R) L/D-reared efemp1^2C-Cas9 (n=16), (S) D/D-reared efemp1+/+ (n=16) and (T) D/D-reared efemp1^2C-Cas9 eyes (n=14), as well as their (U) b-wave amplitude, (V) a-wave amplitude, (W) b-wave implicit time and (X) a-wave implicit time measured after 4 weeks of treatment. Group data are shown as mean±s.e.m. ERGs were measured at −0.83, 0.73 and 1.87 log cd.s.m-2 of stimulus intensities, represented by black blocks of different sizes below graphs; thicker blocks indicate higher intensities. Three-way ANOVA and Fisher's LSD post-hoc tests were performed. Significant main effects of genotypes (G) and interactions between genotypes and rearing conditions (G×E) are indicated in the graphs. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001.