Fig. 4

In silico fate mapping of NMPs and in vivo validation via photoconversion and live imaging (A) In silico fate mapping of the pluripotent early NMP territory. Left: selected territory at the initial time point; middle: intermediate time point showing tracks for selected cells; right: final time point. The red star refers to the RNA velocity in Figure 3A. (B) Quantification of early NMP in silico fate mapping with their progeny in the mesodermal (left) and neural (right) fates (n = 5 animals; Data S1). (C) In silico fate mapping of the late presumptive NMP territory. (D) These cells primarily integrate the mesodermal tissue (n = 4 animals; Supp. Fig. 20a). (E) (Right) Photoconversion of the early presumptive NMP territory at ten hpf. (Left) Distribution of the photoconverted cells and their progeny at 14 hpf. (F) (Right) Segmentation and identification of the photoconverted cells in the area selected in (E) left (rectangle). (Left) Distribution of early photoconverted cells and their progeny with mesodermal and neural fates (n = 5 animals). (G) Right: photoconversion of the late NMP territory at 14 hpf. Left: distribution of the photoconverted cells and their progeny cells at 17 hpf. (H) Segmentation and identification of the photoconverted cells in the area selected in (G) right (rectangle). Right: the distribution of the late photoconverted cells and their progeny (n = 6 animals).