Fig. S4

The in silico reconstruction of well-known embryonic lineage and reconstruction of posterior progenitors mapping, related to Figure 4 (A) In silico fate mapping of a population of forebrain cells. Left: selected territory at the initial time point; middle: intermediate time point showing tracks for selected cells; right: final time point. The purple star ∗ refers to the RNA velocity in Figure 3A. (B) In silico fate mapping of the posterior mesodermal progenitors in the presomitic mesoderm. We observe that these cells integrate into the somites that will then generate muscles. (C) In silico fate mapping of the axial mesodermal posterior progenitors. These cells mostly integrate into the mesoderm (74%). (D) In silico fate mapping of the axial neural posterior progenitors. These cells partly integrate the neural tube (46%). (E) Left: the proportion of the clonal distribution after photoconversion of the different progenitor territories: NMPs early (total number of cells = 75), mesodermal progenitors (total number of cells = 81), neural progenitors (total number of cells = 76), NMPs late (total number of cells = 53). Right: a schematic representation of the different axial progenitor territories.