FIGURE

Fig. 2

ID

ZDB-FIG-241127-2

Publication

Uphoff et al., 2024 - dab2 is required for the scavenging function of lymphatic endothelial cells in the zebrafish meninges

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Fig. 2

Generation of the dab2mu410 allele results in a mRNA null situation. (a) Scheme for the generation of the dab2 promoter knock-out. Two gRNAs (red dotted lines) were designed to remove the TSS and first translated ATG, resulting in a deletion of a 4.6 kb genomic fragment including the dab2 promoter. (b-c) Images of ISH stainings with a dab2 RNA antisense probe. At 24hpf, dab2 is expressed in the DA (red asterisk) and the PCV (blue asterisk) of wild type (b) and heterozygous (c) embryos, whereas no staining is detectable in homozygous mutants (d). ATG, first translated ATG; DA, dorsal aorta; hpf, hours post fertilization; ISH, in situ hybridization; PCV, posterior cardinal vein; TSS, transcription start site.

Expression Data

Gene:	dab2
Fish:	WT dab2^mu410/mu410 dab2^mu410/+
Anatomical Terms:	dorsal aorta posterior cardinal vein
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dab2^mu410/mu410
Observed In:	dorsal aorta posterior cardinal vein
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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