FIGURE 5

Comparison of the miRNA content of unstimulated platelets and the differentially induced PEVs (n = 4; biological replicates representing 16 donors). (a) Principal component analysis of the 541 shared miRNAs show quantitative differences between platelets and PEVs. The US, GPVI, CLEC‐2 and TC PEV miRNAs overlap with each other, and separation is only observed in comparison to the miRNAs of unstimulated platelets. (b) Venn diagram of the DEGs in the US, GPVI, CLEC‐2 and TC PEV miRNAs compared to the platelet miRNAs. Numbers of the differentially up‐ or downregulated miRNAs in PEVs in comparison to the miRNAs of unstimulated platelets are indicated in brackets. (c) Bar graph of the top 10 pathways (p ≤ 0.01) affected by the upregulated miRNAs in the differentially induced PEVs compared to platelets. Analysis was performed using the DESeq2 algorithm and the p‐values were corrected for multiple testing with FDR method. (d) Venn diagram of the upregulated miRNA DEGs of the GPVI, CLEC‐2 and TC PEVs compared to the US PEVs. (e) Venn diagram of the downregulated miRNA DEGs of the GPVI, CLEC‐2 and TC PEVs compared to the US PEVs. DEGs, differentially expressed genes; FDR, false discovery rate; PEVs, platelet‐derived extracellular vesicles.