FIGURE

Figure 3

ID

ZDB-FIG-240930-33

Publication

Han et al., 2024 - Creg1 Regulates Erythroid Development via TGF-β/Smad2-Klf1 Axis in Zebrafish

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Figure 3

Loss of creg1 results in elevated apoptosis of erythroid cells. A) Cell cycle analysis of gata1:dsRed⁺ erythroid cells from WT siblings (left) and creg1^−/− mutants (right) at 2 dpf. Erythroid cell DNA content was measured by flow cytometry via Hoechst 33342 fluorescence intensity. B) Quantification of the percentage of cells in each cell cycle phase from (A) (n=100 embryos per group). C) FACS analyses of apoptotic cell death were performed with gata1:dsRed⁺ erythroid cells from WT siblings (left) and creg1^−/− mutants (right) at 2 dpf using Annexin V/DAPI staining. D) Quantification of the percentage of gata1:dsRed⁺ erythroid cells that are Annexin V‐positive from (C) (n = 100 embryos per group). E) Quantitative PCR analysis of bida and baxa expression in gata1:dsRed⁺ erythroid cells from WT siblings and creg1^−/− mutants at 2 dpf (n = 30 embryos per group). Data shown are the means ± SEM. Statistical significance was calculated using the Student's t‐test. *, p < 0.05; **, p < 0.01; ****, p < 0.0001; ns, not significant.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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