Figure 2

IWR-1 and XAV939-mediated β-catenin pathway inhibition reduces Tg(Hsa.CTGF:nlsmCherry)^ia49 reporter signal. (A,B) Tg(Hsa.CTGF:nlsmCherry)^ia49 embryos were exposed to IWR-1 or XAV939 for 24 h, and the fluorescent reporter expression was documented (A) and quantified at the level of the whole embryo (B) at 48 hpf. mCherry fluorescence levels (B) were analyzed by measuring the integrated density with FIJI software Control IWR (n = 27), IWR treated embryos (n = 28); Control XAW (n = 31), XAW treated embryos (n = 34). (C) Relative expression of ccn2a and ccn2b genes in zebrafish embryos after treatment with XAV939 from 24 to 48 hpf. mRNA levels were normalized to the DMSO-treated controls (dashed line). Scale bar 250 μm Data are presented as mean +/− SEM (Mann−Whitney test (B), Student t-test (C); * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).