PUBLICATION
Wnt/β-Catenin Signaling Regulates Yap/Taz Activity during Embryonic Development in Zebrafish
- Authors
- Astone, M., Tesoriero, C., Schiavone, M., Facchinello, N., Tiso, N., Argenton, F., Vettori, A.
- ID
- ZDB-PUB-240929-21
- Date
- 2024
- Source
- International Journal of Molecular Sciences 25(18): (Journal)
- Registered Authors
- Argenton, Francesco, Astone, Matteo, Facchinello, Nicola, Schiavone, Marco, Tesoriero, Chiara, Tiso, Natascia, Vettori, Andrea
- Keywords
- Hippo, Wnt/β-catenin, Yap/Taz, crosstalk, embryonic development, zebrafish
- MeSH Terms
-
- Animals
- beta Catenin/genetics
- beta Catenin/metabolism
- Zebrafish*/embryology
- Zebrafish*/genetics
- Zebrafish*/metabolism
- Wnt Signaling Pathway*
- Transcriptional Coactivator with PDZ-Binding Motif Proteins/metabolism
- Gene Expression Regulation, Developmental*
- Adaptor Proteins, Signal Transducing/genetics
- Adaptor Proteins, Signal Transducing/metabolism
- Embryonic Development*/genetics
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- YAP-Signaling Proteins*/metabolism
- PubMed
- 39337493 Full text @ Int. J. Mol. Sci.
Citation
Astone, M., Tesoriero, C., Schiavone, M., Facchinello, N., Tiso, N., Argenton, F., Vettori, A. (2024) Wnt/β-Catenin Signaling Regulates Yap/Taz Activity during Embryonic Development in Zebrafish. International Journal of Molecular Sciences. 25(18):.
Abstract
Hippo-YAP/TAZ and Wnt/β-catenin signaling pathways, by controlling proliferation, migration, cell fate, stemness, and apoptosis, are crucial regulators of development and tissue homeostasis. We employed zebrafish embryos as a model system to elucidate in living reporter organisms the crosstalk between the two signaling pathways. Co-expression analysis between the Wnt/β-catenin Tg(7xTCF-Xla.Siam:GFP)ia4 and the Hippo-Yap/Taz Tg(Hsa.CTGF:nlsmCherry)ia49 zebrafish reporter lines revealed shared spatiotemporal expression profiles. These patterns were particularly evident in key developmental regions such as the midbrain-hindbrain boundary (MHB), epidermis, muscles, neural tube, notochord, floorplate, and otic vesicle. To investigate the relationship between the Wnt/β-catenin pathway and Hippo-Yap/Taz signaling in vivo, we conducted a series of experiments employing both pharmacological and genetic strategies. Modulation of the Wnt/β-catenin pathway with IWR-1, XAV939, or BIO resulted in a significant regulation of the Yap/Taz reporter signal, highlighting a clear correlation between β-catenin and Yap/Taz activities. Furthermore, genetic perturbation of the Wnt/β-catenin pathway, by APC inhibition or DKK1 upregulation, elicited evident and robust alteration of Yap/Taz activity. These findings revealed the intricate regulatory mechanisms underlying the crosstalk between the Wnt/β-catenin and Hippo-Yap/Taz signaling, shedding light on their roles in orchestrating developmental processes in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping