FIGURE

Fig. 2

ID

ZDB-FIG-240814-4

Publication

Emmerich et al., 2024 - Large-scale CRISPR screen reveals context-specific genetic regulation of retinal ganglion cell regeneration

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Fig. 2

Single cell transcriptomics of response to RGC ablation. (A) Experimental setup for scRNA-seq time series (12, 24, 48 and 72 hpa) of unablated control (no Mtz) and RGC-ablated (+Mtz) larvae. (B) UMAP of major eye cell types from all timepoints collected. (C) Total number of DEGs identified at each timepoint for MG, progenitor cells, RGCs and other major retinal cell classes. (D) MG subclusters. Subcluster 3 is made up predominantly of ‘activated’ MG cells at 12 hpa (blue). (E) Volcano plot of DEGs in 12 hpa MG subcluster. (F) Select MG DEGs at 12, 24, 48 and 72 hpa. (G) Cross-paradigm comparison of MG DEGs from RGC ablation data, and a pooled NDMA and LD dataset. (H) Top upregulated and downregulated RGC paradigm-specific DEGs relative to pooled NDMA and LD dataset. ACs, amacrine cells; BCs, bipolar cells; HCs, horizontal cells; PR, photoreceptor; RGCs, retinal ganglion cells. *P≤0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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