Fig. 5

Knockdown of lamp2a impairs the proliferation and differentiation capability of HSPCs. (A) Signal of cd41+ HSPCs was significantly reduced in CHT of 3 dpf Tg(cd41:GFP) embryos injected with lamp2a-MO compared with those with control Std-MO in a dose-dependent manner (n = 19–48 embryos per group; scale bar: 200 μm). (B) WISH for c-myb showed that the HSPC signal was significantly reduced in 3 dpf embryos injected with lamp2a-MO compared with Std-MO (n = 28–39 embryos per group; scale bar: 200 μm). (C) WISH for rag1 showed that lymphoid differentiation was significantly reduced in 5 dpf embryos injected with lamp2a-MO compared with Std-MO (n = 51–56 embryos per group; scale bar: 200 μm). (D) WISH for mpx showed that myeloid differentiation was significantly increased in 3 dpf embryos injected with lamp2a-MO compared with Std-MO (n = 29–30 embryos per group; scale bar: 200 μm). (E) WISH for lplastin showed that myeloid differentiation was significantly increased in 3 dpf embryos injected with lamp2a-MO compared with Std-MO (n = 21–22 embryos per group; scale bar: 200 μm). (F) WISH for gata1 showed that erythroid differentiation was significantly increased in 4 dpf embryos injected with lamp2a-MO compared with Std-MO (n = 18–26 embryos per group; scale bar: 200 μm). (G) WISH for hbbe1 showed that erythroid differentiation was significantly increased in 4 dpf embryos injected with lamp2a-MO compared with Std-MO (n = 18–22 embryos per group; scale bar: 200 μm). **p < .01, ***p < .001; Data were shown as mean ± SD; Statistical analysis was performed using a Shapiro–Wilk test for samples with normal distribution and unpaired student's t tests with two tail.