FIGURE

Fig. 3

ID

ZDB-FIG-240802-32

Publication

Labudina et al., 2024 - Cohesin composition and dosage independently affect early development in zebrafish

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Fig. 3

Bulk RNA-seq analyses of Rad21- and Stag2b-deficient tailbuds. (A) Schematic of progenitor cells and specialized tissues in the zebrafish tailbud. The zebrafish tailbud consists of two pools of bipotent progenitors: neuromesodermal progenitors (NMPs) and midline progenitor cells (MPCs). The dashed line shows the location of tailbud excision for RNA-seq. (B) Principal component analysis of gene expression in wild-type and cohesin-deficient tailbuds at the 16-somite stage. Genotypes are distinguished by color: wild-type samples are displayed in purple, rad21−/− in blue, rad21+/− in green, and stag2b−/− in yellow. (C-E) MA [M (log ratio) and A (mean average) scales] plots displaying changes in gene expression in rad21−/− (C), rad21+/− (D) and stag2b−/− (E) compared with wild-type tailbuds. Each dot represents a gene, with colored dots indicating those with significant (5% FDR) changes in expression; 7520 genes were dysregulated in rad21−/− tailbuds (3678 up- and 3842 downregulated), and 5144 genes were dysregulated in rad21+/− tailbuds (2645 up- and 2499 downregulated). In contrast, stag2b−/− tailbuds had substantially fewer dysregulated genes (2054: 866 up- and 1188 downregulated).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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