Fig. 5 Supplemental 4
- ID
- ZDB-FIG-240801-23
- Publication
- Nakayama et al., 2021 - A zebrafish embryo screen utilizing gastrulation identifies the HTR2C inhibitor pizotifen as a suppressor of EMT-mediated metastasis
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Expression of Snail and Twist1 was examined by western blotting in the MCF7 cells (left); GAPDH loading control is shown (bottom). GAPDH band is the same as one from Figure 4C since GAPDH bands in Figure 4C (bottom left) and Figure 5—figure supplement 4 (bottom left) were obtained in the same experiment. Protein expression levels of Twist1 in either vehicle- or pizotifen-treated MDA-MB-231 cells are shown (middle): GAPDH loading control is shown (bottom). Protein expression levels of Snail and Twist1 of 4T1 primary tumors from either vehicle- or pizotifen-treated mice are shown (right); luciferase is used as loading control for whole cell. Luciferase control was obtained in the same experiment from Figure 5C. |