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Fig. 5 Supplemental 4 Expression of Snail and Twist1 was examined by western blotting in the MCF7 cells (left); GAPDH loading control is shown (bottom). GAPDH band is the same as one from Figure 4C since GAPDH bands in Figure 4C (bottom left) and Figure 5—figure supplement 4 (bottom left) were obtained in the same experiment. Protein expression levels of Twist1 in either vehicle- or pizotifen-treated MDA-MB-231 cells are shown (middle): GAPDH loading control is shown (bottom). Protein expression levels of Snail and Twist1 of 4T1 primary tumors from either vehicle- or pizotifen-treated mice are shown (right); luciferase is used as loading control for whole cell. Luciferase control was obtained in the same experiment from Figure 5C.