FIGURE

Fig. 4

ID

ZDB-FIG-240730-4

Publication

Roychaudhury et al., 2024 - SRPK3 Is Essential for Cognitive and Ocular Development in Humans and Zebrafish, Explaining X-Linked Intellectual Disability

Other Figures

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Fig. 4

Generation of srpk3 zebrafish knockout (KO) line. (A) Disruption of protein domains in KO zebrafish while wild-type (WT) zebrafish show intact srpk3 protein. Protein kinase domain 1, PK-1; protein kinase domain 2, PK-2. (B) KO zebrafish fail to develop swim bladder inflation at 5 dpf when compared to WT siblings (red arrow). (C) Expression analysis of neuronal markers (her4, th, islet1) and srpk3 at 72hpf. Scale bars; 200 μm. (D, E) Reduced expression level of srpk3 mRNA in KO zebrafish. Three adult brains for each group were used in qRT-PCR experiments (n = 3 for WT and n = 3 for KO).

Expression Data

Genes:	her4.1 isl1a srpk3 th
Fish:	WT srpk3^{ck243a/ck243a} srpk3^{ck243b/ck243b}
Anatomical Terms:	brain brain dopaminergic neuron brain motor neuron brain neuron
Stage Range:	Protruding-mouth to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	srpk3^{ck243a/ck243a} srpk3^{ck243b/ck243b}
Observed In:	brain swim bladder
Stage Range:	Protruding-mouth to Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Ann. Neurol.