Fig. 7

SB225002-treated neutrophils attenuate mpeg+ presence at the injury site. (A – D) Representative maximal projections displaying mpeg+ and tnfa+ cells in the injured spinal cord at 8 and 24 hpi in DMSO- and SB225002-treated larvae. Scale bar, 30 μm. Individual panels for tnfa (A’ − D’) and mpeg (A’’ − D’’) highlight double-positive cells. (E) Quantification of mpeg+ cells in the injured spinal cord (n = 24–31, performed as two independent experiments). Two-way ANOVA followed by Tukey’s multiple comparison test revealed a statistically significant difference between DMSO- and SB225002-treated larvae at 8 (p < 0.0001), 24 (p < 0.0001) and 48 hpi (p = 0.0315). Additionally, significant differences were observed in DMSO-treated larvae between 8 and 24 hpi (p = 0.0079) and between 24 and 48 hpi (p < 0.0001) (grey pounds), with no significant difference in SB225002-treated larvae between 8 and 24 hpi (p = 0.2161) and 24 and 48 hpi (p = 0.3950). (F) Quantification of the number of mpeg+ tnfa+ cells in the injured spinal cord (n = 24–31, performed as two independent experiments). Two-way ANOVA followed by Tukey’s multiple comparison test showed a statistically significant decrease between DMSO- and SB225002-treated larvae at 8 (p < 0.0001) and 24 hpi (p = 0.002), with no significant difference at 48 hpi (p = 0.9991). (G) Quantification of the proportion of mpeg+ tnfa+ cells over the total mpeg+ population (n = 24–31, performed as two independent experiments). Two-way ANOVA followed by Tukey’s multiple comparison test showing statistically significant increase between DMSO- and SB225002-treated larvae at 24 (p < 0.0001) and 48 hpi (p < 0.0001). Additionally, a significant decrease was observed in the DMSO-treated group between 8 and 24 hpi (p = 0.0014) and 24 and 48 hpi (p < 0.0001). (H – O) Representative maximal projections illustrate mpeg+ cells in transgenic Rac2 and Rac2D57N DMSO- and SB225002-treated larvae at 8 and 24 hpi. Scale bar, 30 μm. (P) Quantification of mpeg+ cells in the injured spinal cord in Rac2 and Rac2D57N larvae (n = 18–23, performed as two independent experiments). Two-way ANOVA followed by Tukey’s multiple comparison test showed a significant decrease between DMSO- and SB225002-treated larvae at 8 (p < 0.0001) and 24 hpi (p < 0.0001) in Rac2 larvae and at 8 (p = 0.0039) and 24 hpi (p < 0.0001) in Rac2D57N larvae. Additionally, significant increases were observed between 8 and 24 hpi in DMSO- Rac2 (p < 0.0001) and Rac2D57N (p < 0.0001) and SB225002- Rac2D57N treated larvae (p = 0.0002). No statistically significant difference in SB225002 Rac2 larvae between 8 and 24 hpi was observed (p = 0.0707).