PUBLICATION
Neutrophil immune profile guides spinal cord regeneration in zebrafish
- Authors
- de Sena-Tomás, C., Rebola Lameira, L., Rebocho da Costa, M., Naique Taborda, P., Laborde, A., Orger, M., de Oliveira, S., Saúde, L.
- ID
- ZDB-PUB-240627-17
- Date
- 2024
- Source
- Brain, behavior, and immunity 120: 514-531 (Journal)
- Registered Authors
- de Oliveira, Sofia, Orger, Mike
- Keywords
- Macrophage, Microglia, Neutrophils, Reverse migration, Spinal cord regeneration, Zebrafish
- Datasets
- GEO:GSE271113
- MeSH Terms
-
- Animals
- Inflammation/immunology
- Inflammation/metabolism
- Macrophages/immunology
- Macrophages/metabolism
- Microglia/immunology
- Microglia/metabolism
- Neutrophil Infiltration/physiology
- Neutrophils*/immunology
- Neutrophils*/metabolism
- Receptors, CXCR4/metabolism
- Spinal Cord*/immunology
- Spinal Cord*/metabolism
- Spinal Cord Injuries*/immunology
- Spinal Cord Injuries*/metabolism
- Spinal Cord Regeneration*/physiology
- Tumor Necrosis Factor-alpha/metabolism
- Zebrafish*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 38925414 Full text @ Brain Behav. Immun.
Citation
de Sena-Tomás, C., Rebola Lameira, L., Rebocho da Costa, M., Naique Taborda, P., Laborde, A., Orger, M., de Oliveira, S., Saúde, L. (2024) Neutrophil immune profile guides spinal cord regeneration in zebrafish. Brain, behavior, and immunity. 120:514-531.
Abstract
Spinal cord injury triggers a strong innate inflammatory response in both non-regenerative mammals and regenerative zebrafish. Neutrophils are the first immune population to be recruited to the injury site. Yet, their role in the repair process, particularly in a regenerative context, remains largely unknown. Here, we show that, following rapid recruitment to the injured spinal cord, neutrophils mostly reverse migrate throughout the zebrafish body. In addition, promoting neutrophil inflammation resolution by inhibiting Cxcr4 boosts cellular and functional regeneration. Neutrophil-specific RNA-seq analysis reveals an enhanced activation state that correlates with a transient increase in tnf-α expression in macrophage/microglia populations. Conversely, blocking neutrophil recruitment through Cxcr1/2 inhibition diminishes the presence of macrophage/microglia at the injury site and impairs spinal cord regeneration. Altogether, these findings provide new insights into the role of neutrophils in spinal cord regeneration, emphasizing the significant impact of their immune profile on the outcome of the repair process.
Genes / Markers
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Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping