Fig. 4

Metabolic changes in cox7a1−/− adult zebrafish skeletal muscle (A) Heatmap depicting proteins related to different metabolic pathways upregulated or downregulated in cox7a1−/− adult zebrafish skeletal muscle compared with wild-type siblings according to p value < 0.05 and a mean log fold-change (LFC) of ±1 (n = 3 replicates per group, each replicate consisting of a tissue pool from 5 animals). Scale reveals normalized expression levels. Proteins with superscripts 1 and 2 indicate that they were related to more than one pathway (2 or 3, respectively). Bar indicates expression levels. (B) Blood glucose levels in overnight fasting condition, 1 h post feeding, and 2 h post feeding (n = 10 males per genotype). Shown are mean ± SD. Two-way ANOVA, data point at 1 h post feeding, p = 0.988. (C) Representative image of skeletal muscle PAS staining in whole zebrafish paraffin sections (n = 10 both sexes per genotype). Scale bars, 100 μm. (D) Metabolites detected using NMR spectroscopy in cox7a1−/− adult zebrafish skeletal muscle compared with wild-type siblings (n = 5 biological replicates, males, and one technical replicate). Data represented as mean ± SD. Unpaired t test. a.u., arbitrary units; ppm, parts per million. (E) Schematic representation of metabolic pathways affected in cox7a1−/− adult zebrafish skeletal muscle. Significantly downregulated enzymes are shown in blue, and significantly upregulated enzymes are shown in red. Blue squares, significantly downregulated metabolites. Red squares, significantly upregulated metabolites. Gray squares, detected metabolites that remain constant in the compare conditions.