Figure 1

Increase in frailty index with age. (A) Total length (cm) and (B) total weight (g) increased in an age-dependent manner. (C) BMI (g/cm²) increased significantly in 30- and 60-month groups. (D) Total distance (cm) decreased significantly in 30- and 60-month groups with respect to the younger groups. (E) Maximum speed (cm/s) underwent a tendency to decrease, being significant in the oldest group with respect to the 2 and 10 month-groups. (F) Mean speed (cm/s) showed a prominent decrease in the 30-month group and remained in the 60-month group relative to the young groups. (G) The groups of 2 and 10 months had a frailty index of 0. However, at 30 months, we observed an increase, which became significant at 60 months. Data are presented as mean ± SEM (n = 8–10 animals/group). * p < 0.05 vs. 2 mo; ** p < 0.01 vs. 2 mo; *** p < 0.001 vs. 2 mo; # p < 0.05 vs. 10 mo; ## p < 0.01 vs. 10 mo; ### p < 0.001 vs. 10 mo; $ p < 0.05 vs. 30 mo; $$ p < 0.01 vs. 30 mo; $$$ p < 0.001 vs. 30 mo. One-way ANOVA with a Tukey’s post hoc test.