FIGURE

Figure 3—figure supplement 2.

ID

ZDB-FIG-240617-49

Publication

Noonan et al., 2024 - A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma

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Figure 3—figure supplement 2.

qPCR shows that TIE:EGFP⁺ macrophages express higher levels of melanocyte markers mitfa and sox10 (compared to TIE:EGFP^- macrophages), validating that macrophages preferentially phagocytose TGFb responsive melanoma cells.

Two Tg(mitfa:BRAF^V600E);p53^-/-;mitfa^-/-;MCR:MCS tumors expressing TIE:EGFP and mpeg:mCherry were dissected and TIE:EGFP⁺;mpeg:mCherry⁺ macrophages and TIE:EGFP^-;mpeg:mCherry⁺ macrophages were isolated by FACS. RNA was extracted, reverse transcribed, and qPCR was performed. Graphed here are individual replicate values and their mean, and error bars represent standard deviation. Two-tailed unpaired t-test was used to calculate significance. n=2 fish with three technical replicates.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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