FIGURE

Figure 3

ID

ZDB-FIG-240613-44

Publication

D'Gama et al., 2024 - Ciliogenesis defects after neurulation impact brain development and neuronal activity in larval zebrafish

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Figure 3

Cerebellar defects in 4 days old elipsa mutants

(A1–B1) Cell nucleus stained using dapi in control (A1) and elipsa mutant (B1). Differences in morphology indicated by yellow lines. n = 7 controls and 7 mutants. (A2–B2) Larvae immunostained with anti-acetylated tubulin to stain axonal bundles (indicated with white arrow) in control (A2) and elipsa mutant (B2). n = 7 controls and 7 mutants.

(C and D) Larvae immunostained with the presynaptic vesicle marker SV2 in control (C) and elipsa mutant (D). n = 6 controls and 6 mutants (E and F) Expression of glutamatergic cells in Tg(vglut2a:dsRed) transgenic zebrafish in control (E) and elipsa mutant (F). n = 8 controls and 8 mutants (G and H) Immunostaining of cerebellar Purkinje neurons using anti-parvalbumin antibody in control (G) and elipsa mutant (H). n = 10 controls and 8 mutants. ∗ indicates nonspecific signal from blood cells and vasculature. Number of Purkinje cells in control (mean ± standard deviation): 83.87 ± 5.22 cells, in mutants: 58 ± 6.8 cells, p-value 4,6965E-06 according to Student’s t test. Teo, Optic Tectum; CCe, Corpus Cerebelli; CC, Crista cerebralis.

Expression Data

Gene:	DsRed
Antibodies:	Ab-SV2 Ab1-pvalb Ab1-tuba
Fish:	WT traf3ip1^tp49d/tp49d nns9Tg traf3ip1^tp49d/tp49d; nns9Tg
Anatomical Terms:	cerebellum axon cerebellum glutamatergic neuron cerebellum presynapse Purkinje cell
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	traf3ip1^tp49d/tp49d traf3ip1^tp49d/tp49d; nns9Tg
Observed In:	cerebellum cerebellum axon cerebellum glutamatergic neuron cerebellum presynapse Purkinje cell
Stage:	Day 4

Phenotype Detail

Acknowledgments

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