Figure 3

Figure 3

Cerebellar defects in 4 days old elipsa mutants

(A1–B1) Cell nucleus stained using dapi in control (A1) and elipsa mutant (B1). Differences in morphology indicated by yellow lines. n = 7 controls and 7 mutants. (A2–B2) Larvae immunostained with anti-acetylated tubulin to stain axonal bundles (indicated with white arrow) in control (A2) and elipsa mutant (B2). n = 7 controls and 7 mutants.

(C and D) Larvae immunostained with the presynaptic vesicle marker SV2 in control (C) and elipsa mutant (D). n = 6 controls and 6 mutants (E and F) Expression of glutamatergic cells in Tg(vglut2a:dsRed) transgenic zebrafish in control (E) and elipsa mutant (F). n = 8 controls and 8 mutants (G and H) Immunostaining of cerebellar Purkinje neurons using anti-parvalbumin antibody in control (G) and elipsa mutant (H). n = 10 controls and 8 mutants. ∗ indicates nonspecific signal from blood cells and vasculature. Number of Purkinje cells in control (mean ± standard deviation): 83.87 ± 5.22 cells, in mutants: 58 ± 6.8 cells, p-value 4,6965E-06 according to Student’s t test. Teo, Optic Tectum; CCe, Corpus Cerebelli; CC, Crista cerebralis.