FIGURE

Fig. 3

ID

ZDB-FIG-240603-84

Publication

Breuer et al., 2024 - CHD7 regulates craniofacial cartilage development via controlling HTR2B expression

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Fig. 3

Osteoblasts and Osteoclasts in early development and adults. (A) RT-qPCR of osteoblast (runx2a, runx2b, sp7/osterix, bglap and postna) and osteoclast related genes (ctsk) screened at 9 dpf and 12 dpf in chd7+/+ and chd7-/-larvae. (B) Immunohistochemistry of osteoblast marker Runx2 (red) and nuclei maker Hoechst (blue) in 3dpf chd7+/+ (n = 5) and chd7-/- (n = 5) larvae. Scale bar = 60 μm. (C) Alkaline Phosphatase staining (ALP; blue) for osteoblasts in craniofacial region of 9dpf larvae (n = 10 for both genotypes). Scale bar = 200 μm. (D) Alkaline Phosphatase staining (ALP; blue) for osteoblasts in adult scales and quantitative analysis determined by signal/area ratio (n = 6 for chd7+/+ and n = 8 for chd7-/-). Scale bar = 100 μm. (E) Immunohistochemistry of osteoclast marker cathepsin K (red) and nuclei maker Hoechst (blue) in 3dpf chd7+/+ (n = 5) and chd7-/- (n = 5) larvae. Scale bar = 60 μm. (F) TRAP staining for osteoclasts in craniofacial region of 9dpf larvae (n = 10 for both genotypes). Scale bar = 100 μm. (G) TRAP staining for osteoclasts in adult scales and quantitative analysis determined by signal/area ratio (n = 10 for both genotypes). Scale bar = 100 μm. *p < 0.05; **p < 0.01 ***p < 0.001; ***p < 0.0001.

Expression Data

Genes:	bglap ctsk postna runx2a runx2b sp7
Antibody:	Ab3-runx2
Fish:	WT chd7^iaf17/iaf17
Anatomical Terms:	osteoblast whole organism
Stage Range:	Protruding-mouth to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	chd7^iaf17/iaf17
Observed In:	head osteoblast head osteoclast osteoblast scale osteoblast scale osteoclast whole organism
Stage Range:	Protruding-mouth to Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Bone Miner. Res.