FIGURE

FIGURE 1

ID

ZDB-FIG-240529-65

Publication

Giarmarco et al., 2024 - Spatial detection of mitochondrial DNA and RNA in tissues

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FIGURE 1

(A) Simplified schematic of protein synthesis from mtDNA. mtRNA transcripts from the mtDNA coding strand are processed and translated into protein; transcripts from the noncoding strand are typically degraded. Accordingly, the RNAscope™ Dr-MT-ND5 “coding” probe reports both mtRNA and mtDNA, while the “noncoding” probe reports primarily mtDNA. (B) Workflow for mtDNA and mtRNA detection using RNAscope™ and IHC. RNAscope™ steps were carried out using the manufacturer’s protocol for the Fluorescent Multiplex v2 kit, with modifications listed in the methods section. (C) Images of zebrafish outer retina stained with a control probe against Polr2a nuclear-encoded mRNA, and a control probe against bacterial-encoded DapB mRNA. RNase treatment eliminates Polr2a mRNA signal.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol