FIGURE 1

FIGURE 1

(A) Simplified schematic of protein synthesis from mtDNA. mtRNA transcripts from the mtDNA coding strand are processed and translated into protein; transcripts from the noncoding strand are typically degraded. Accordingly, the RNAscope™ Dr-MT-ND5 “coding” probe reports both mtRNA and mtDNA, while the “noncoding” probe reports primarily mtDNA. (B) Workflow for mtDNA and mtRNA detection using RNAscope™ and IHC. RNAscope™ steps were carried out using the manufacturer’s protocol for the Fluorescent Multiplex v2 kit, with modifications listed in the methods section. (C) Images of zebrafish outer retina stained with a control probe against Polr2a nuclear-encoded mRNA, and a control probe against bacterial-encoded DapB mRNA. RNase treatment eliminates Polr2a mRNA signal.