FIGURE

FIGURE 3

ID

ZDB-FIG-240529-67

Publication

Giarmarco et al., 2024 - Spatial detection of mitochondrial DNA and RNA in tissues

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FIGURE 3

(A) Sample images of zebrafish photoreceptor mitochondrial clusters from 9 a.m. to 11 p.m. labeled with RNAscope™ MT-ND5 coding or noncoding probes. RNase was used to determine signal contributions of RNA for each probe. All sections were counterstained for nuclei and mitochondria via IHC. Cyan arrowheads and yellow arrows indicate examples of single rod and cone mitochondrial clusters used for quantification, respectively. Green–probe (ISH), magenta–mitochondria (IHC), blue–nuclei. (B) Quantification of signals from coding and noncoding probes at 9 a.m. and 11 p.m., including RNase conditions. (C) Ratios of mtDNA to gDNA measured using RT-qPCR from whole zebrafish retinas at 9 a.m. and 11 p.m.. For all graphs, error bars represent standard error of the mean; p values determined using a Mann-Whitney test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol