Fig. 2
- ID
- ZDB-FIG-240528-39
- Publication
- Fetsko et al., 2024 - IL-1β disrupts the initiation of blood-brain barrier development by inhibiting endothelial Wnt/β-catenin signaling
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Il-1? expression disrupts CNS angiogenesis in the transgenic CNS/Il-1? model (A) Representative confocal microscopy images showing dose-dependent effects of Dox on brain vascular development. CNS/Il-1?, kdrl:EGFP embryos were untreated (No Dox) or treated with Dox (0.1, 1.0, or 10.0 ?g/mL) at 6 hpf and then imaged at 52 hpf (dorsal view; anterior left). Scale bars are 100 ?m. (B) Quantification of the number of CtAs in CNS/Il-1? embryos treated with Dox (0, 0.1, 1.0, or 10.0 ?g/mL) (n = 5). (C) Representative confocal microscopy images showing whole embryo vasculature in CNS/Il-1?, kdrl:EGFP embryos at 2 dpf (lateral view; dorsal top; anterior left). CNS/Il-1? embryos were either untreated (No Dox) or treated (Dox; 10.0 ?g/mL) at 6 hpf. Note the loss of CNS vasculature with Dox treatment but no effect on trunk vasculature with Dox treatment. Scale bar is 100 ?m. (D) Quantification of the number of intersegmental vessels (ISVs) at 52 hpf in CNS/Il-1? embryos either untreated (No Dox) or treated (10.0 ?g/mL Dox) (n = 4). Error bars in B and D represent means ± SEM (??p < 0.01; ???p < 0.001; no label = not significant). |