FIGURE

Fig. 6

ID

ZDB-FIG-240517-32

Publication

Zhu et al., 2024 - Allelic heterogeneity of TTNtv cardiomyopathy can be modeled in adult zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

ulk1a mutants repair defective autophagic flux in both embryonic and adult stages of ttntv-A mutants. (A) Schematics of ulk1ae6 generated by MMEJ sgRNA. Red indicates deleted nucleotides. * indicates the nonsense codons. (B and C) Quantification of the autophagosome puncta in 2 dpf embryos either with or without BafA1 treatment and the autolysosome/autophagosome ratio of BafA1-untreated groups. ulk1a+/? can reduce the basal autophagosome level of dA/dA and can recover the autophagic flux affected by dA/dA. One-way ANOVA was applied. (D?F) Representative Western blot and quantification of ulk1a and atg7 from 3-month zebrafish hearts. One-way ANOVA was applied. (G and H) Representative Western blot and quantification of LC3-II from zebrafish hearts either with or without BafA1 treatment at 3 months. Independent samples t test was applied to compare 2 groups. Data are presented as mean � SD.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ JCI Insight