FIGURE

Fig. 4

ID

ZDB-FIG-240502-46

Publication

Pomreinke et al., 2024 - Zebrafish nampt-a mutants are viable despite perturbed primitive hematopoiesis

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Fig. 4

Expression of hemangioblast/endothelial and erythrocyte maturation markers in nampt-a loss-of-function conditions. nampt-a^+/+ (+ / +) and nampt-a^t10pm/t10pm (-/-) embryos were injected with nampt-a morpholino (MO). Embryos were assayed at 1 dpf for the expression of the hemangioblast/endothelial marker kdrl (A) and the erythrocyte markers gata1a (B) and klf1 (C) by qRT-PCR. Fold change was calculated using the ∆∆C_t method [26]. Each point represents one biological sample of ten embryos pooled for RNA extraction. Each colored line represents the average from separate experimental trial days with the black line representing the average from all trials. For statistical analysis, the Kruskal–Wallis non-parametric test was applied followed by Dunn’s multiple comparison test to determine p-values. D Effect size (η²) for the Kruskal–Wallis test to compare each group

Expression Data

Genes:	gata1a kdrl klf1
Fish:	cz3331Tg cz3331Tg + MO1-nampt1 nampt1^t10pm/t10pm; cz3331Tg nampt1^t10pm/t10pm; cz3331Tg + MO1-nampt1
Knockdown Reagent:	MO1-nampt1
Anatomical Term:	whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cz3331Tg + MO1-nampt1 nampt1^t10pm/t10pm; cz3331Tg nampt1^t10pm/t10pm; cz3331Tg + MO1-nampt1
Knockdown Reagent:	MO1-nampt1
Observed In:	whole organism
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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