FIGURE

Fig. 4

ID
ZDB-FIG-240502-46
Publication
Pomreinke et al., 2024 - Zebrafish nampt-a mutants are viable despite perturbed primitive hematopoiesis
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Fig. 4

Expression of hemangioblast/endothelial and erythrocyte maturation markers in nampt-a loss-of-function conditions. nampt-a+/+ (+ / +) and nampt-at10pm/t10pm (-/-) embryos were injected with nampt-a morpholino (MO). Embryos were assayed at 1 dpf for the expression of the hemangioblast/endothelial marker kdrl (A) and the erythrocyte markers gata1a (B) and klf1 (C) by qRT-PCR. Fold change was calculated using the ∆∆Ct method [26]. Each point represents one biological sample of ten embryos pooled for RNA extraction. Each colored line represents the average from separate experimental trial days with the black line representing the average from all trials. For statistical analysis, the Kruskal–Wallis non-parametric test was applied followed by Dunn’s multiple comparison test to determine p-values. D Effect size (η2) for the Kruskal–Wallis test to compare each group
Expression Data
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Prim-5

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Phenotype Detail
Acknowledgments
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