FIGURE

Figure 2

ID

ZDB-FIG-240429-30

Publication

Brenet et al., 2024 - Microglia Mitigate Neuronal Activation in a Zebrafish Model of Dravet Syndrome

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Figure 2

Microglia dynamics in scn1Lab-KD larvae. Confocal time-lapse analysis of microglia dynamics in the brains of 4 dpf not-injected Tg[mpeg1:mCherryF] control (A₁,A₂) and Tg[mpeg1:mCherryF]; scn1Lab-KD larvae (B₁–B₄), and merged images of two-time points highlighting the increased move of microglia cell bodies in scn1Lab-KD larvae (C₂) compared to that observed in WT controls during a 36-min period (C₁) (0′: cyan, 36′: magenta, merge: white). Quantification of microglia cell body displacements (D) and mean displacement speed (E) over a 60-min period, in 4 dpf scn1Lab-KD larvae (N = 6; n = 55) and WT controls (N = 4; n = 46). (F) Microglial cell tracking over a 60-min period, in 4 dpf control (F₁) and scn1Lab-KD (F₂) larva brain. The scale bar is 20 µm in all images. N = number of larvae and n = number of microglial cells. All images were acquired with an SP8 Leica laser scanning confocal equipped with a 40×/water 1.1 objective. All data are represented as the mean ± sem. p-values were determined using the Mann–Whitney test. ****, p < 0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gl23Tg/gl23Tg + MO2-scn1lab scn1lab^s552/s552; gl23Tg/gl23Tg
Knockdown Reagent:	MO2-scn1lab
Observed In:	microglial cell locomotory behavior
Stage:	Day 4

Phenotype Detail

Acknowledgments

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