FIGURE

Fig. 6

ID

ZDB-FIG-240426-22

Publication

Tran et al., 2024 - Caffeine-induced protein kinase A activation restores cognitive deficits induced by sleep deprivation by regulating O-GlcNAc cycling in adult zebrafish

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Fig. 6

Effects of caffeine, rolipram, and H89 on O-GlcNAc cycling in Neuro2a cells. Neuro2a cells were treated with 0, 5, 10, and 50 μM caffeine (A), rolipram (B), or H89 (C) for 24 h. Total cell lysates were prepared and subjected to Western blot using specific antibodies. Representative Western blot images and quantification graphs of O-GlcNAc, OGT, and OGA are presented (n = 3 replicates/group). One-way ANOVA with Tukey’s post hoc multiple comparison test was used to determine the statistical significance of Western blot quantification (*P < 0.05, **P < 0.01, ***P < 0.001 vs. Control). Caff, caffeine; OGA, O-GlcNAcase; OGT, O-GlcNAc transferase; Rol, rolipram.

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Am. J. Physiol. Cell Physiol.