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Fig. 6 Effects of caffeine, rolipram, and H89 on O-GlcNAc cycling in Neuro2a cells. Neuro2a cells were treated with 0, 5, 10, and 50 μM caffeine (A), rolipram (B), or H89 (C) for 24 h. Total cell lysates were prepared and subjected to Western blot using specific antibodies. Representative Western blot images and quantification graphs of O-GlcNAc, OGT, and OGA are presented (n = 3 replicates/group). One-way ANOVA with Tukey’s post hoc multiple comparison test was used to determine the statistical significance of Western blot quantification (*P < 0.05, **P < 0.01, ***P < 0.001 vs. Control). Caff, caffeine; OGA, O-GlcNAcase; OGT, O-GlcNAc transferase; Rol, rolipram.