FIGURE

Fig. 6

ID

ZDB-FIG-240424-48

Publication

Xu et al., 2024 - Vibrio cholerae arrests intestinal epithelial proliferation through T6SS-dependent activation of the bone morphogenetic protein pathway

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Fig. 6

Vc T6SS induces BMP activation and limits cell proliferation in the zebrafish intestine (A) Intestines of TL zebrafish larvae uninfected or Vc infected for 24 h with DNA stained by Hoechst in blue and EdU+ cells in cyan. Scale bars: 200 μm. (B) Quantification of EdU+ cells per gut. (C) Immunohistochemical images of sagittal posterior intestinal sections from adult TL zebrafish uninfected or Vc infected for 24 h stained for PCNA. Arrows indicate epithelial damages marked by disorganized nuclei and shedding of epithelial cells. Scale bars: 50 μm. (D) Percentage of intestinal epithelial cells that are PCNA+ in adult fish guts. (E) Posterior intestines from TL zebrafish larvae uninfected or Vc infected for 24 h with DNA marked by Hoechst in blue and pSMAD3 in magenta. Scale bars: 100 μm. (F) Quantification of pSMAD3+ cells per gut. Each dot represents a measurement from a single fish intestine. p values are calculated using the significance tests indicated in the tables.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TL
Condition:	bacterial treatment by exposure to environment: Vibrio cholerae
Observed In:	intestinal epithelial cell intestinal epithelium intestinal epithelium cell population proliferation
Stage Range:	Days 7-13 to Adult

Phenotype Detail

Acknowledgments

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