FIGURE

Fig. 2

ID

ZDB-FIG-240419-82

Publication

Huppertz et al., 2024 - Recording physiological history of cells with chemical labeling

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Recording of GPCR signaling using split-HaloTag. (A) Scheme of split-HaloTag–based GPCR recorder. Fusion of Hpep to βArr2 and cpHaloΔ to a GPCR leads to labeling of split-HaloTag after ligand binding and downstream βArr2 recruitment. (B) Fluorescence micrographs of human embryonic kidney (HEK) 293 cells coexpressing Hpep1-βArr2-T2A-mTagBFP2 and D2R-cpHaloΔ-GGS-EGFP treated with or without quinpirole (100 μM, 1 hour) in the presence of CPY-CA (200 nM). The color scale bar represents the ratio of fluorescence intensities. Scale bars are 50 μm. (C) Quantification of D2R-cpHaloΔ-EGFP labeling (N > 500 cells; p < 0.0001; Welch’s t test). Error bars indicate median and 25 and 75% quantiles. D2Roff, D2R lacking cpHaloΔ; D2Ron, D2R fused to cpHaloTag154-156. (D) Labeling of HEK293 cells coexpressing D2R-cpHaloΔ and Hpep-βArr2 in the presence of varying concentrations of dopamine or quinpirole. Data are shown as mean ± SEM. A sigmoidal model was applied to determine EC50 values.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Science