PUBLICATION
Recording physiological history of cells with chemical labeling
- Authors
- Huppertz, M.C., Wilhelm, J., Grenier, V., Schneider, M.W., Falt, T., Porzberg, N., Hausmann, D., Hoffmann, D.C., Hai, L., Tarnawski, M., Pino, G., Slanchev, K., Kolb, I., Acuna, C., Fenk, L.M., Baier, H., Hiblot, J., Johnsson, K.
- ID
- ZDB-PUB-240223-5
- Date
- 2024
- Source
- Science (New York, N.Y.) 383: 890897890-897 (Journal)
- Registered Authors
- Baier, Herwig, Schneider, Martin, Slanchev, Krasimir
- Keywords
- none
- MeSH Terms
-
- Animals
- Calcium*
- Cell Physiological Phenomena*
- Cells*/chemistry
- Coloring Agents
- Gene Expression Profiling
- Protein Interaction Domains and Motifs
- Staining and Labeling*
- Zebrafish
- PubMed
- 38386755 Full text @ Science
Citation
Huppertz, M.C., Wilhelm, J., Grenier, V., Schneider, M.W., Falt, T., Porzberg, N., Hausmann, D., Hoffmann, D.C., Hai, L., Tarnawski, M., Pino, G., Slanchev, K., Kolb, I., Acuna, C., Fenk, L.M., Baier, H., Hiblot, J., Johnsson, K. (2024) Recording physiological history of cells with chemical labeling. Science (New York, N.Y.). 383:890897890-897.
Abstract
Recordings of the physiological history of cells provide insights into biological processes, yet obtaining such recordings is a challenge. To address this, we introduce a method to record transient cellular events for later analysis. We designed proteins that become labeled in the presence of both a specific cellular activity and a fluorescent substrate. The recording period is set by the presence of the substrate, whereas the cellular activity controls the degree of the labeling. The use of distinguishable substrates enabled the recording of successive periods of activity. We recorded protein-protein interactions, G protein-coupled receptor activation, and increases in intracellular calcium. Recordings of elevated calcium levels allowed selections of cells from heterogeneous populations for transcriptomic analysis and tracking of neuronal activities in flies and zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping