Cell-type marker genes for 30dpf ZW and ZZ gonads. (A) UMAP plot for 30dpf gonads combining ZW and ZZ cells. (B) Cells originating from ZW (gold) or ZZ (blue) gonads, showing several clusters with sex-specific genotypes. (C,D). Germ cells marked by dazl and ddx4 (vasa) expression. (E,F). Support cells (granulosa and Sertoli) or their precursors marked by gsdf and lhx9 expression. (G,H). Steriogenic cells (theca, Leydig) or their precursors labeled with inhbaa and nr5a1b expression. (I,J). Stroma/interstitial cells marked by col1a1a and pcolcea expresseion. (K) White blood cells identified by coro1a expression. (L) Vasculature labeled with si:dkeyp-97a10.2 expression. Abbreviations: g, germ cells; oo, oocytes; si, stroma/interstitial; sd, steroidogenic; su, support; v, vascular; w, white blood cells.
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