Germ cell development in the merged analysis of both ZW and ZZ cells at both 19 and 30dpf. (A) UMAP isolating germ cell clusters. (B) Germ cells marked by age (red, 19dpf; blue, 30dpf). (C) Germ cells marked by sex genotype (red, ZW; blue, ZZ). (D)ddx4(vasa) including all four conditions. (E)ddx4 showing only ZW cells at 19dpf. Insert in lower left of the panel shows ddx4 expression in the ZW germ cell cluster 19c17 in the analysis of 19dpf cells only (see Figure 3). (F)ddx4, only ZW cells at 30dpf. (G)ddx4, only ZZ cells at 19dpf. Insert in lower left of the panel shows ddx4 expression in the ZZ germ cell cluster 19c17 from the analysis of 19dpf cells only. (H)ddx4, only ZZ cells at 30dpf. (I)dnd1 ZW cells only at both 19dpf and 30dpf. (J)dnd1 ZZ cells only at both 19dpf and 30dpf. (K)fkbp6, both ZW and ZZ cells only at 19dpf. (L)fkbp6, both ZW and ZZ cells only at 30dpf. (M)piwil2, ZW and ZZ cells at 19dpf. (N)piwil2, ZW and ZZ cells at 30dpf. (O)bmp15, ZW cells at both 19dpf and 30dpf. (P)bmp15, ZZ cells at both 19dpf and 30dpf. (Q)buc, ZW cells at both 19dpf and 30dpf. (R). buc, ZZ cells at both 19dpf and 30dpf. (S)zp2.2, ZW cells at both 19dpf and 30dpf. (T)zp2.2, ZZ cells at both 19dpf and 30dpf. (U)dmrt1, ZW cells at both 19dpf and 30dpf. (V)dmrt1, ZZ cells at both 19dpf and 30dpf. (W)spo11, ZW cells at both 19dpf and 30dpf. (X)spo11, ZZ cells at both 19dpf and 30dpf. (Y)hormad1, ZW cells at both 19dpf and 30dpf. (Z)hormad1, ZZ cells at both 19dpf and 30dpf. (AA) rad21l1, ZW cells at both 19dpf and 30dpf. (BB) rad21l1, ZZ cells at both 19dpf and 30dpf. (CC)smc1b, ZW cells at both 19dpf and 30dpf. (DD) smc1b, ZZ cells at both 19dpf and 30dpf. (EE) sycp3, ZW cells at both 19dpf and 30dpf. (FF)sycp3 ZZ, cells at both 19dpf and 30dpf.
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