Steroidogenesis. (A). An abbreviated pathway of steroidogenesis (after (Tenugu et al., 2021)). (B)nr5a1b expression. The left panel shows expression in the entire dataset. The insert shows cluster demarcation for the most relevant steroidogenic enzymes. Subsequent panels show, at the top 1930c21, the support cell precursor and granulosa cell cluster and at the bottom the clusters corresponding to the insert in the left panel. Displayed from left to right are the enlargements of all four conditions: 19dpf ZW gonads, 30dpf ZW gonads, 19dpf ZZ gonads, and 30dpf ZZ gonads. Cluster numbers are indicated. (C) cyp11a1.2 displayed as explained for nr5a1b. (D)cyp17a1. (E)cyp19a1a. (F). hsd17b1. (G)hsd17b3. (H)cyp11c1. (I)hsd11b2. Abbreviations: L, Leydig cells; th, theca cells.
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