Stromal/interstitial clusters. (A) Expression of col1a1a defines stromal/interstitial cells (Liu et al., 2022). The left large panel combines cells of all four conditions for the complete dataset and the four smaller panels to the right show an enlargement of the col1a1a-expressing domain for each condition independently. At 19dpf, ZW and ZZ expression patterns are similar but at 30dpf, some clusters are sex-genotype specific. (B) The UMAP is in the left large panel; the middle panel shows the col1a1a-expressing region for samples separated by sex genotype (red, ZW; blue, ZZ); and the right panel shows samples separated by age (red, 19dpf; blue, 30dpf). (C)acta2 expression, with panels as described for (A). (D)panx3 expression in the 30dpf ZZ-specific cluster 1930c32. (E)efn2b expression, indicating likely arterial endothelial cells in 1930c22 boxed in the large panel and expanded in the four condition-specific panels to the right. (F)lyve1a expression, a marker of lymphatics, labeling 1930c22.
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